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ATCC murine aortic vsmcs
GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. <t>(A)</t> <t>MOVAS</t> cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Murine Aortic Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. (A) MOVAS cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.

Journal: Frontiers in Cell and Developmental Biology

Article Title: GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification

doi: 10.3389/fcell.2020.589761

Figure Lengend Snippet: GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. (A) MOVAS cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.

Article Snippet: Murine aortic VSMCs (MOVAS-1 CRL-2797, ATCC, Manassas, VA, United States) were maintained in DMEM 6546 medium supplemented with 10% FCS, 100 IU/ml penicillin, 100 μg/ml streptomycin, 4 mM glutamine, and 200 μg/ml geneticin (G418) at 37°C in a humidified 5% CO 2 atmosphere.

Techniques: Incubation, Staining